Design of S–S bond containing maleimide-conjugated closo -dodecaborate (SSMID): identification of unique modification sites on albumin and investigation of intracellular uptake

Authors:   Satomu Ishii,Shinichi Sato,Hiroya Asami,Tomoko Hasegawa,Jun-Ya Kohno,Hiroyuki Nakamura,
Journal: Organic & Biomolecular Chemistry
https://doi.org/10.1039/C9OB00584F
Abstract: An S–S bond containing maleimide-conjugated closo-dodecaborate (SSMID) was synthesised for identification of albumin binding sites. Three Lys residues, Lys221, Lys413 and Lys431, were identified as SSMID modification sites in addition to Cys34 in bovin serum albumin (BSA). Fluorescent-labelled MID-BSA was found to be accumulated in the cytosol of HeLa cells.