2011 Article Biodistribution ICNCT14 In-vitro Studies Year 2011

BPA uptake does not correlate with LAT1 and Ki67 expressions in tumor samples (results of EORTC trial 11001)

Applied Radiation and Isotopes, 2011

Authors:   Andrea Wittig,Sien-Yi Sheu-Grabellus,Laurence Collette,Raymond Moss,Lorenzo Brualla,Wolfgang Sauerwein,
Journal: Applied Radiation and Isotopes
Abstract: The system l-amino acid transporter (LAT1) imports p-boronophenylalanine (BPA) mainly into proliferating cells. This study investigates in three tumor entities whether the proportion of tumor cells expressing LAT1 and/or Ki67 correlates with BPA uptake. Tumors were analyzed for 10B concentration with prompt gamma-ray spectroscopy and for Ki67 and LAT1 expressions with immunohistochemical methods. The proportion of LAT1-expressing cells was much higher (5–90%) than that of Ki67-expressing cells (0–20%) and cells expressing both Ki67 and LAT1 (0–5%). Neither LAT1 nor Ki67 expression predicted BPA uptake.