2012 Article Biodistribution Drug Delivery System In-vivo Studies Year 2012

Boron delivery with liposomes for boron neutron capture therapy (BNCT): Biodistribution studies in an experimental model of oral cancer demonstrating therapeutic potential

Radiation and Environmental Biophysics, 2012

Authors:   Elisa Heber,Peter Kueffer,Mark Lee,M. Hawthorne,Marcela Garabalino,Ana Molinari,David Nigg,William Bauer,Andrea Hughes,Emiliano Pozzi,Verónica Trivillin,Amanda Schwint,
Journal: Radiation and Environmental Biophysics
Abstract: Boron neutron capture therapy (BNCT) combines selective accumulation of10B carriers in tumor tissue with subsequent neutron irradiation. We previously demonstrated the therapeutic efficacy of BNCT in the hamster cheek pouch oral cancer model. Optimization of BNCT depends largely on improving boron targeting to tumor cells. Seekingto maximize the potential of BNCT for the treatment for head and neck cancer, the aim of the present study was to perform boron biodistribution studies in the oral cancer model employing two different liposome formulations that were previously tested for a different pathology, i.e., in experimental mammary carcinoma in BALB/c mice: (1) MAC: liposomes incorporating K[nido-7-CH3(CH2)15-7,8-C 2B9 H11] in the bilayer membrane and encapsulating a hypertonic buffer, administered intravenously at 6 mg B per kg body weight, and (2) MAC-TAC: liposomes incorporating K[nido-7-CH 3(CH2)15-7,8-C2B9H 11] in the bilayer membrane and encapsulating a concentrated aqueous solution of the hydrophilic species Na3 [ae-B20H 17NH3], administered intravenously at 18 mg B per kg body weight. Samples of tumor, precancerous and normal pouch tissue, spleen, liver, kidney, and blood were taken at different times postadministration and processed to measure boron content by inductively coupled plasma mass spectrometry. No ostensible clinical toxic effects were observed with the selected formulations. Both MAC and MAC-TAC delivered boron selectively to tumor tissue. Absolute tumor values for MAC-TAC peaked to 66.6 ± 16.1 ppm at 48 h and to 43.9 ± 17.6 ppm at 54 h with very favorable ratios of tumor boron relative to precancerous and normal tissue, making these protocols particularly worthy of radiobiological assessment. Boron concentration values obtained would result in therapeutic BNCT doses in tumor without exceeding radiotolerance in precancerous/normal tissue at the thermal neutron facility at RA-3. © Springer-Verlag 2012.